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琼脂糖凝胶电泳
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==备注== #50×TAE Buffer 配制方法 ##称量Tris 242 g和Na<sub>2</sub>EDTA·2H<sub>2</sub>O 37.2 g于1 L烧杯中; ##向烧杯中加入约600 ml去离子水,充分搅拌溶解; ##加入57.1 ml的冰乙酸,充分搅拌; ##加去离子水定容至1 L后,室温保存。 Na+离子0.01-0.04 M,浓度太低时电泳速度变慢;太高会造成过大的电流使胶发热甚至熔化;EDTA 1-2 M,目的是螯合Mg2+,防止电泳时激活DNA酶,以及防止Mg2+离子与核酸生成沉淀。 #长链DNA:电泳缓冲液还需要加Mg2+,稳定DNA #loading buffer ##避免DNA上浮; ##与样品的比例按照buffer的说明书 ##含有溴酚蓝,电泳时在最前沿 #核酸染料gold view ##需要避光,在加入后的制胶和电泳过程中,都关灯或黑色塑料袋遮光进行: </div>
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